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Structured Review

Proteintech bad
Bad, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+bad/BAD+Antibody/pm41872189-64-50-52
Average 95 stars, based on 81 article reviews
bad - by Bioz Stars, 2026-09
95/100 stars

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Related Articles

Membrane:

Article Title: The long noncoding RNA MIR4435-2HG enhances the migration, promotion, and glycolysis of nonsmall cell lung cancer cells by targeting the miR-371a-5p/SOX2/PI3K/Akt axis
Article Snippet: The separated proteins were subsequently transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore). .. The membrane was blocked with 5% nonfat milk at room temperature for 1 h and then incubated with primary antibodies, including anti-SOX2 (ab92494, Abcam, 1:1000), anti-CDK2 (Cat No. 10122-1-AP, Proteintech, 1:1000), anti-CDK4 (Cat No. 11026-1-AP, Proteintech, 1:1000), anti-CyclinD1 (Cat No. 26939-1-AP, Proteintech, 1:1000), anti-CyclinE1 (Cat No. 11554-1-AP, Proteintech, 1:1000), anti-Bax (Cat No. 50599-2-Ig, Proteintech, 1:1000), anti-Bcl2 (Cat No. 68103-1-Ig, Proteintech, 1:1000), anti-Bad (Cat No. 10435-1-AP, Proteintech, 1:1000), anti-LDHA (ab52488, Abcam, 1:1000), anti-GLUT1 (ab115730, Abcam, 1:1000), anti-HK-1 (Cat No. 19662-1-AP, Proteintech, 1:1000), anti-HK-2 (Cat No. 22029-1-AP, Proteintech, 1:1000), anti-PKM1 (Cat No. 15821-1-AP, Proteintech, 1:1000), anti-PKM2 (Cat No. 15822-1-AP, Proteintech, 1:1000), anti-E-cadherin (Cat No. 20874-1-AP, Proteintech, 1:1000), anti-Vimentin (Cat No. 10366-1-AP, Proteintech, 1:1000), anti-N-cadherin (Cat No. 22018-1-AP, Proteintech, 1:1000), anti-p-PI3K p85(ab138364, Abcam, 1:1000), anti-PI3K p85 (ab191606, Abcam, 1:1500), anti-p-Akt (ab38449, Abcam, 1:1000), anti-Akt (ab8805, Abcam, 1:2000), anti-p-mTOR (ab109268, Abcam, 1:1000), anti-mTOR (ab32028, Abcam, 1:2000), anti-GAPDH (ab8245, Abcam, 1:3000) overnight at 4°C. .. After washing with PBS, the membrane was incubated with horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary antibody (ab6721, Abcam, 1:1000) at room temperature for 2 h. Finally, enhanced chemiluminescence (P0018S, Beyond Biotechnology) was used for color imaging.

Incubation:

Article Title: The long noncoding RNA MIR4435-2HG enhances the migration, promotion, and glycolysis of nonsmall cell lung cancer cells by targeting the miR-371a-5p/SOX2/PI3K/Akt axis
Article Snippet: The separated proteins were subsequently transferred to polyvinylidene fluoride (PVDF) membranes (EMD Millipore). .. The membrane was blocked with 5% nonfat milk at room temperature for 1 h and then incubated with primary antibodies, including anti-SOX2 (ab92494, Abcam, 1:1000), anti-CDK2 (Cat No. 10122-1-AP, Proteintech, 1:1000), anti-CDK4 (Cat No. 11026-1-AP, Proteintech, 1:1000), anti-CyclinD1 (Cat No. 26939-1-AP, Proteintech, 1:1000), anti-CyclinE1 (Cat No. 11554-1-AP, Proteintech, 1:1000), anti-Bax (Cat No. 50599-2-Ig, Proteintech, 1:1000), anti-Bcl2 (Cat No. 68103-1-Ig, Proteintech, 1:1000), anti-Bad (Cat No. 10435-1-AP, Proteintech, 1:1000), anti-LDHA (ab52488, Abcam, 1:1000), anti-GLUT1 (ab115730, Abcam, 1:1000), anti-HK-1 (Cat No. 19662-1-AP, Proteintech, 1:1000), anti-HK-2 (Cat No. 22029-1-AP, Proteintech, 1:1000), anti-PKM1 (Cat No. 15821-1-AP, Proteintech, 1:1000), anti-PKM2 (Cat No. 15822-1-AP, Proteintech, 1:1000), anti-E-cadherin (Cat No. 20874-1-AP, Proteintech, 1:1000), anti-Vimentin (Cat No. 10366-1-AP, Proteintech, 1:1000), anti-N-cadherin (Cat No. 22018-1-AP, Proteintech, 1:1000), anti-p-PI3K p85(ab138364, Abcam, 1:1000), anti-PI3K p85 (ab191606, Abcam, 1:1500), anti-p-Akt (ab38449, Abcam, 1:1000), anti-Akt (ab8805, Abcam, 1:2000), anti-p-mTOR (ab109268, Abcam, 1:1000), anti-mTOR (ab32028, Abcam, 1:2000), anti-GAPDH (ab8245, Abcam, 1:3000) overnight at 4°C. .. After washing with PBS, the membrane was incubated with horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG secondary antibody (ab6721, Abcam, 1:1000) at room temperature for 2 h. Finally, enhanced chemiluminescence (P0018S, Beyond Biotechnology) was used for color imaging.



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